🧠 New pre-print by Wiesner et al. (2025) shows non-#synaptic #exocytosis directly from the #axon shaft, regulated by the submembrane periodic skeleton. Using #superresolution #imaging and live assays (#HiLo (VAMP2-pHluorin), #SIM, and correlative two-color #SMLM/ #STORM) they reveal that #axons can release vesicles outside classical #synapses, expanding how we understand #neuronal communication and #AxonalSignaling.
#SMLM
Congratulations to Prof. Jin Lu, now at the National Center for Nanoscience, whose work on #singlemolecule #electrochemical imaging is now online in Chemical Science. Happy New Year!
#SMLM #WashU #WUSTL @chemicalscience #ChemicalScience
"Single-Molecule Electrochemical Imaging Resolves the Midpoint Potentials of Individual Fluorophores on Nanoporous Antimony-Doped Tin Oxide"
Chemical Science, 2024
https://doi.org/10.1039/D3SC05293A
High-end #automated 3D #OpenSource multi-color single-molecule localization #microscopy:
https://doi.org/10.1101/2023.10.23.563122
#DIYbio #lab #instruments #SMLM #microscope
Very proud of this work of Clément Cabriel, finally published in Nature Photonics!
Event-based vision sensors are here to stay and conquer your hearts and #SMLM microscopes.
Article here: https://www.nature.com/articles/s41566-023-01308-8
Full text access here: https://rdcu.be/dpQhX
Are there any microscopists hiding here?
Adding :AddBiophysicist to this post in order to get in the "Biophysicists on Mastodon" list...
Cost-efficient #OpenSource #laser engine for #microscopy:
-405 nm, 488 nm & 638 nm and optionally a 561 nm diode-pumped solid-state laser
-agitated multimode fiber to suppress speckles
https://doi.org/10.1364/boe.380815
#DIYbio #hackerspace #lab #instruments #SMLM #microscope #imaging
Simple, economic & robust rail-based #OpenSource setup for multicolor super-resolution localization #microscopy:
https://pubs.acs.org/doi/10.1021/acs.jpca.3c01351
#DIYbio #lab #instruments #microscope #SMLM #imaging
An #OpenSource, high resolution, #automated #fluorescence #microscope:
-reaches single molecule sensitivity
-single particle tracking and #SMLM super-resolution microscopy experiments in cells
https://doi.org/10.1101/2023.05.31.542706
#DIYbio #lab #instruments #optics #imaging
Team achieves Ångström-resolution #fluorescence #microscopy.
https://phys.org/news/2023-05-team-ngstrm-resolution-fluorescence-microscopy.html
Analyzing complex #SMLM images of synthetic materials like nanoparticles is crucial for drug delivery advancements. @crisleft_ uses #MachineLearning to assess nanoparticles characterization and classification for diverse applications #SuperResolution 🔬 @NEUBIAS_ Symposium
Super-cool work from our clever colleagues at Cambridge -- whole-cell #3D #singlemolecule #lightfield imaging! #SMLM
High-density volumetric super-resolution microscopy
bioRxiv 2023.05.02.539032;
doi: https://doi.org/10.1101/2023.05.02.539032
Two days left to talk with our representative Nicolaas about #smlm + cluster analysis with Huygens, and all our other image restoration and analysis solutions. #TIM2023 #imaging #microscopy #imageanalysis
New post on FocalPlane
Our preprints list this week focusses on research that uses microscopy tools to answer questions in #cellbio and #devbio, featuring #volumeEM, #lightsheet, #TIRF, #SMLM, #CLEM and #bioimageanalysis!
"A framework for evaluating the performance of SMLM cluster analysis algorithms."
A critical contribution, #SMLM (#SingleMoleculeLocalizationMicroscopy) is highly confounded data, without validation, large scale (1e6+ 3D points), with #fuzzy localizations, non-specific labelling, multiple localizations, ... . Resolving protein complexes/ structures from such data is non-trivial to say the least. Robust evaluation of such methods (and p-choices) is welcome.
Belated intro as people migrate here in earnest:
I'm a neuroscientist currently postdocing at Stanford. My research is focused on how nanometer-scale protein organization controls cellular signal transduction at synapses and beyond. I use a range of techniques, including #cryoET , #mousegenetics, and #smlm to visualize and manipulate this organization with molecular resolution.
Also into dogs, coffee, science tattoos, and objectively dumb humour 👋
🤩🔬 Heya everyone! Together with #Abbelight, we're thinking about organizing a #Microscopy one-day workshop at IGC (near Lisbon, March 31st). Just before the April 2nd start of #FOM2023 in Porto. #Scientific talks, #SMLM discussions and #SAFe360 hands-on. Interested in joining?
Yesterday, I gave a lecture about #SMLM as part of Bryan Millis's #Microscopy Education Program "Widening the Lens"🔬 sponsored by #cziscience #imagingthefuture - You can find my presentation on #figshare - Feel free to reach out in case of questions - Enjoy 😀👉 https://figshare.com/articles/presentation/Single-Molecule_Localization_Microscopy_Theoretical_Basis_and_Practical_Guide/19902511
#superresolution #nanoscopy #womeninscience #imageanalysis #imaging
Are you unfamiliar with the terms #STED, #PALM, #STORM, #SMLM, #MINFLUX, & #MINSTED, but would you like to learn more about these #superresolution #microscopy technologies🔬? If so, then you should check out #StefanHell's #Lindau presentation 🤓 Enjoy👉 https://mediatheque.lindau-nobel.org/recordings/39775
#introduction #superresolution #lightsheet #nanoscopy #smlm
Hi everyone!
My name is Ulrike, & I'm a #Physicist, Optical Scientist & #DataScientist passionate about community building/engagement, outreach, & teaching!
Currently, I'm working as an Optical Scientist & Project Team Lead at the #CarlZeiss AG (#teamzeiss, #zeiss) in #Oberkochen. Here, I'm part of the Corporate Research & Technology unit working on the latest optical trends for #imaging, #microscopy, & #metrology.
Let's connect!
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