Lmst

How can you use your free netScope Cloud account to share #microscopy files with other #pathologists or #vets?
netScope makes it easy to share complex WSI files, such as #MRXS or #fluorescence, with macOS users.
This is your guide.
https://www.netscope.de/en/cloud
(For personal sharing use a Cloud Premium Subscription.)

The interior of the peridioles of Nidula niveotomentosa (a birds nest fungus) have surprising green fluorescence in 365 nanometer UV light. Photographed yesterday in Mendocino County, California.

Sliced in half with a sharp razor blade.

#fungi #mushrooms #mycology #uvphotography #fluorescence

Fluorescent Friday

https://youtu.be/Boz2sfnyIFo

I have always been intrigued by how and why some creatures fluoresce above and below the waterline.

Biofluorescence is the absorption and reemission of light from living organisms. Fluorescent organisms have proteins built into their skin or other tissues that absorb energy from light and reemit it as a different colors. This results in a wonderful display of color.

In the ocean, blue light penetrates through the water, where some organisms absorb that energy, and then emit light at a lower energy in colors such as green or red. This article from Dive Photo Guide dives into the science of fluorescence as it applies to underwater photography.

Scuba divers need special yellow filters to see the reemitted light. In addition to my normal camera set-up, I used a blue light, and yellow filters on my camera lens and on my mask. Below are the additional items I used to capture the video above.

Sealife Sea Dragon Mini Fluoro Light
- This light came with yellow filter for a scuba diver’s mask.
- Since this was my first experience with fluoro diving and I was not sure how it would work. I selected a lower end mini model and was pleased with the results. However, if I have the opportunity to upgrade, I would go with the higher model that has a wider beam range. For non-photographers/videographers, the mini would suit them well.
Tiffen Yellow Filter
- The yellow barrier filter blocked any reflected excitation light and transmit only the fluorescence from the subject.
- Basically, allowed me to record exactly what I was seeing through the mask filter.
- It’s a wet lens, so I just attached it to the front of my underwater camera housing.

I shot this video off the house reef at Six Senses Laamu Resort during our first night dive there. The Laamu Atoll is located in the Maldives.

Overall, I was amazed by the color captured during the night dive. I will plan to try again on my next night dive. Hopefully, I can schedule the night dive around coral spawning. This is the time when corals colonies reproduce by releasing their eggs and sperm all at the same time. However, with a global pandemic, I’m not sure if I’ll be able to coordinate this year.

#Biofluorescence #Biofluorescent #coral #fluorescence #fluorescent #fluoroDiving #Nature #NightDive #NightDiving

Biofluorescent coral on a night dive in the maldives

Haven't seen this sample of yooperlite glow this strongly before! The 20W 365 nm UV LED flashlight is *really* making it shine! #geology #science #fluorescence #rocks

A rock on a wooden table. Parts of the rock are fluorescing bright yellow-orange due to the UV light that is shining on it. Some visible light is being emitted from the UV flashlight and so you can see a blue glow around the rock indicating where the beam is at.

A picture of the same rock not fluorescing. It is not shining at all and looks quite dull.

Just came across #FPbase, a community-driven database for #fluorescent #proteins and #spectral properties 🌈. Very useful for selecting reporters, comparing #excitation/ #emission #spectra, planning multi-color #imaging 🔬, and avoiding spectral overlap.

🌍 https://www.fpbase.org/about/

#Microscopy #Fluorescence #Neuroscience #OpenScience

#FluorescenceFriday

Ridley Creek State Park, PA

I really need to go to this creek tomorrow or Sunday…

#FlyFishing #Nature #IPA #Beer #Creek #Hiking #autumn #Fluorescence #Photography

A creek flows through the woods, where trees with bright yellow and red leaves line the far bank. Their colors shimmer like fluorescence in the moving water, partially veiled by branches from the near bank, yet still vivid and bright.

An early 20th century uranium glass plate glowing under UV illumination. It's only slightly radioactive: 180 CPM from my 1987 Soviet G-M tube, where background is under 30 CPM.

#uv #photography #fluorescence #uranium

Top view of a green glass plate sitting on a plain grey carpet

The plate has 12 sides with rounded edges. Stamped into the back of the glass are bunches of grapes sitting on grape leaves. The centre has a multipoint rosette. The whole plate is glowing violently green, as if illuminated from within.

The carpet background is illuminated faintly purple, from a small UV LED light source held above the scene and out of shot.

Last week to subscribe for our FREE Webinar Series on "Mastering Colocalization Analysis"; from raw image to scientific results in minutes. Reserve your seat now!
http://svi.nl/webinarinvitation
#imaging #microscopy #cellbiology #fluorescence #imageanalysis #colocalization

Le documentaire "La Vie en Fluo", sur @artefr est non seulement magnifique visuellement mais aussi extrêmement intéressant pour apporter un éclairage (hoho) nouveau sur un nombre très important de comportements animaux.

https://www.arte.tv/fr/videos/113555-001-A/la-vie-en-fluo/

#Arte #fluorescence #fluo #animaux #documentaire

#VikingSpectrophotometer: A home-built, simple & cost-efficient #OpenSource #absorption & #fluorescence #spectrophotometer for #education in #chemistry:
https://pubs.acs.org/doi/10.1021/acs.jchemed.2c00679
#DIYbio #lab #instruments #spectroscopy #EDU #MINT #STEM #school

Teach your #microscope how to print: Low-cost rapid-iteration #OpenSource #microfabrication for #biology:

- no cleanroom required
- replace SU-8 #photoresist & silicon wafers by #3Dprinting #resin & microscope slides
- #fluorescence microscope-based maskless #photolithography
- achieve µm-scale precision across cm-sized areas

https://doi.org/10.1039/D5LC00181A
#DIYbio #lab #instruments #microfluidics